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Image Search Results
Journal: Journal of immunology (Baltimore, Md. : 1950)
Article Title: Mycobacterium tuberculosis membrane vesicles inhibit T cell activation
doi: 10.4049/jimmunol.1601199
Figure Lengend Snippet: (A) EVs were purified from uninfected or M. tuberculosis-infected macrophages to produce Ctr EVs or Inf EVs, respectively. EVs (5 µg protein content) were assessed by western blot for host CD9 and CD63 (left) and M. tuberculosis lipoglycans (right, top band represents LAM and bottom band represents LM). (B) CD4+ T cells from spleens of C57BL/6 mice were treated for 1 h with Inf EVs or Ctr EVs (100 µg/ml EV protein concentration) and activated for 24 h with 1 µg/ml plate bound anti-CD3ε and 1 µg/ml soluble anti-CD28 in the continued presence of the EVs. IL-2 was measured by ELISA. Data are shown as mean ± SD of triplicate samples from one representative experiment (n>3 experiments). Student t test with Welch’s correction was used to assess statistical significance (*≤0.05).
Article Snippet: Abs (monoclonal unless otherwise stated) included polyclonal rabbit anti- M. tuberculosis (Gen-Way Biotech, San Diego, CA), CS-35 anti-LAM (hybridoma from BEI Resources, Manassas, VA), polyclonal rabbit anti-GRAIL (Thermo Fisher, Grand Island, NY 14072), EM-04
Techniques: Purification, Infection, Western Blot, Protein Concentration, Enzyme-linked Immunosorbent Assay